Locust root nodule fungus|358900 |BNCC

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Rhizobium sophoriradicis

Culture medium	BNCC360815
Description	Nitrogen fixing medium
Composition	KH₂PO₄:0.2g, K₂HPO₄:0.8g, MgSO₄.7H₂O:0.2g, CaSO₄.2H₂O:0.1g, FeCl₃:Trace, Na₂MoO₄.2H₂O:Trace, Yeast Extract:0.5g, Mannitol:20.0g, Agar:15.0g, pH:7.2(25℃)
Growth conditions	28 ℃; 24-48 hours; aerobic;
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Transparent
Sharing mode	Public welfare sharing