Bacillus licheniformis|266600 |BNCC

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Bacillus licheniformis

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C, 18-24h, aerobic
Subculture procedure	① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it, and subsequently crush it with forceps; ③ Pipette 0.5mL liquid medium into the lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all test tubes and plates under the specified conditions; the strain is ready for use once growth appears.
Storage conditions	2-8°C
Safety level	1
morphology	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: Grayish-white Elevation: Low Surface: Roughly wrinkled Texture: Dry and viscous, G+ (blue-violet), cocci, Purity: Pure, Produces mucus and spores
Sharing mode	Public welfare sharing