BeNa Culture Collection
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| Culture medium | BNCC352256 |
| Description | Czapek Dox Agar |
| Composition | NaNO3:3.0g, K2HPO4:1.0g, MgSO4:0.5g, KCl:0.5g, FeSO4:0.01g, Sucrose:30.0g, Agar:15.0g |
| Growth conditions | 28 ℃; 5-7 days; aerobic; |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Filamentous fungi have obvious colonies on Czapek's agar medium, with white, dense and flat hyphae that vigorously spread and grow, producing a large number of white spores |
| Sharing mode | Public welfare sharing |
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