Pan-cultured Bacillus subtilis|336994 |BNCC

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Paracoccus pantotrophus

Culture medium	BNCC352232
Description	Tryptic Soy Broth
Composition	Tryptone:17.0g, Soy Peptone:3.0g, NaCl:5.0g, K₂HPO₄:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃)
Growth conditions	30°C; 18-24h; aerobic
Subculture procedure	① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Immediately crush with forceps; ③ Pipette 0.5 mL sterile water into freeze-dried tube. Thoroughly dissolve bacterial powder, then inoculate 2 plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular with neat, opaque edges; yellow upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; G- (red); coccus; purity: pure
Sharing mode	Public welfare sharing