Pan-cultured Bacillus subtilis|336994 |BNCC

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Paracoccus pantotrophus

Subculture procedure	① Prepare two plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Immediately crush with forceps; ③ Pipette 0.5 mL sterile water into freeze-dried tube. Thoroughly dissolve bacterial powder, then inoculate 2 plates (200 μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	30°C; 18-24h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular with neat, opaque edges; yellow upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; G- (red); coccus; purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name: Paracoccus pantotrophus

2. BNCC No.:336994

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, Tryptone soybean agar (TSA): tryptone (casein trypsin digest) 15.0g, soybean peptone (soybean meal papain digest) 5.0g, sodium chloride 5.0g, agar 15.0g,pH7.3±0.2. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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