Bacillus lipolyticus|337016 |BNCC

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Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to shatter it; break into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Growth conditions	30°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm, circular, with neat edges; opaque, yellow on top, centrally convex, surface shiny, moist texture, easily lifted; Gram-negative (red), bacillus
Sharing mode	Public welfare sharing

1.Description

1. BNCC No.:337016

2. Biosafety level:4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, Nutritious gravy agar: peptone 10g, beef paste 3.0g,NaCl 5g, agar 15g, distilled water 1L,pH7.0-7.2.

2. Atmosphere: aerobic

3, Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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