Bacillus licheniformis|337190 |BNCC

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Bacillus licheniformis

Subculture procedure	① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then promptly add sterile water to rupture. Crush ampoule with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. Fully dissolve bacterial powder, then inoculate 2 plates (200μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge.
Growth conditions	30°C; 24-48h; aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular with neat margins; opaque, grayish-white upper surface; centrally convex; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); bacilli; purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name: Bacilluslicheniformis
2. BNCC No.:337190
3. Biosafety level: 4
2. Storage conditions
Storage of freezed dried ampoule and agar slant at 2°C to 8°C
3. Growth Conditions
1. Nutritional gravy agar: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add 5mg MnSO4·H2O when culturing Bacillus,which is beneficial to spore production. pH7.0. 121 ℃,15min.
2. Atmosphere: aerobic
3. Temperature: 30 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization.

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