Lactobacillus plantarum (Lactobacillus plantarum var. plantarum)|337212 |BNCC

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Lactobacillus plantarum

Subculture procedure	① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in biosafety cabinet, flame top with alcohol lamp, then quickly add sterile water to break it; shatter with forceps; ③ Pipette 0.5 mL liquid medium into freeze-dried vial, dissolve completely, then return to liquid test tube and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Growth conditions	37°C; 24-48h; microaerophilic (3%-5% O₂, 10% CO₂)
Storage conditions	2-8°C
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Neat Transparency: Opaque Color: Milky white Elevation: Central dome Surface: Bright and smooth Texture: Moist and easily lifted
Sharing mode	Public welfare sharing

1.Description

1. Name:Lactobacillusplantarum

2. BNCC No.:337212

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. MRS medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1000mL. pH6.2±0.2. 121 ℃,15min.

2. Atmosphere:micro aerobic

3. Temperature: 37 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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