Mycoplasma pulses|386815 |BNCC

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Mycoplasma pulmonis

Culture medium	BNCC367740
Description	Mycoplasma Broth without Glucose
Composition	Porcine gastric digest:10g, Beef extract:5g, Yeast extract:5g, NaCl:2.5g, Phenol red:0.02g, Horse serum:200ml, Penicillin:800, 000 units, pH:7.6±0.2(25℃)
Growth conditions	37 ℃; 5 weeks; aerobic;
Subculture procedure	① Prepare 4-5 tubes containing 4.5mL of prepared mycoplasma culture medium (with added serum and penicillin); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the first liquid test tube; ④ Take 0.5mL of suspension from the first test tube and transfer it to the second test tube; ⑤ Repeat the above operation and dilute the gradient to the last tube (note: one tube needs to be left as a blank control). ⑥ Cultivate under the above culture conditions, and the strain can be used once it grows (the color of the culture tube changes from red to yellow).
Storage conditions	2-8 ℃
Safety level	1
morphology	Color change
Sharing mode	Public welfare sharing