BeNa Culture Collection
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| Culture medium | BNCC376171 |
| Description | Mycoplasma arginine Broth |
| Composition | Porcine gastric digest:10g, Beef extract:5g, Yeast extract:5g, NaCl:2.5g, Glucose:1.0g, L-Arginine(C6H14N4O2):2.0g, Phenol red:0.02g, Horse serum:200ml, Penicillin:800, 000 units, pH:7.1±0.2(25℃) |
| Growth conditions | 37 ℃; |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | Liquid nitrogen |
| Safety level | 1 |
| morphology | After 20 days of cultivation, the color of the culture tube changed from red to yellow |
| Sharing mode | Public welfare sharing |
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