BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 3-4 days; microaerophilic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open plates in a biosafety cabinet after surface disinfection; ③ Pipette 1-3 ml sterile water onto plates; use a sterile spreader to scrape bacterial film from surface to create suspension; ④ Pipette suspension with Pasteur pipette onto fresh plates and spread evenly; ⑤ Incubate plates under specified conditions. (Keep plates moist during incubation. If plates dry out, open them in a safety cabinet and add appropriate amounts of tryptic soy broth.) |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Pure |
| Sharing mode | Public welfare sharing |
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Human herpesvirus 2 nucleic acid reference (Heat inactivated) (Strongly positive)(Human herpesvirus 2 nucleic acid reference (Heat inactivated) (Strongly positive))
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