BeNa Culture Collection
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| Culture medium | BNCC353703 |
| Description | 2216E agar |
| Composition | Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC6H5O7):0.1g, KBr:0.08g, NaCl:19.45g, MgCl2:5.98g, Na2SO4:3.24g, CaCl2:1.8g, KCl:0.55g, Na2CO3:0.16g, Na2NO3:0.0016g, Na2HPO4:0.008g, SrCl2:0.034g, H3BO3:0.022g, Na2SiO3:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃) |
| Growth conditions | 22-25°C; 24-48h; aerobic |
| Subculture procedure | ① Thaw Frozen vials in a 30°C water bath, vigorously shake to dissolve; ② Disinfect vial exterior with alcohol swab, then transfer to safety cabinet for handling; ③ Unscrew cap, aspirate entire thawed solution, and inoculate 1-2 agar plates (approx. 200μL per Agar plate); ④ Spread evenly and incubate under specified conditions. |
| Storage conditions | -80°C |
| Safety level | 1 |
| morphology | Pure |
| Sharing mode | Public welfare sharing |
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