Pseudomonas aeruginosa|BNCC186070 |BNCC

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Pseudomonas aeruginosa

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic;
Growth characteristics	G-bacilli, lysine decarboxylase, ornithine decarboxylase were negative, oxidase, arginine dihydrolase were positive, indole, V-P test was negative, fermentation of glucose, fructose, no fermentation of lactose, no H2S. Produce pyogenin. Specialized aerobic, optimum pH7.4 ~ 7.6.
Storage conditions	2-8 ℃
Safety level	2
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white Protuberance: Middle convex surface: Bright and smooth Texture: Wet and easy to pick
Model strain	no
application	Research, quality control, standard control bacteria for cosmetic testing, "Quality Requirements for GB 4789.28 Media and Reagents" standard strains
Sharing mode	Public welfare sharing

Pseudomonas aeruginosa

No.: 186070

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: Freeze-dried tube for 24 months

Biosafety level: 2, handle in biosafety cabinet

Notes: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37°C, aerobic, nutrient agar/gravy medium (NA/NB). Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121 ℃, 15min.

Recovery steps:

(1)Prepare a flask of NB liquid media or two NA agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 37℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a NA agar plate evenly, then put the plates in incubator at 37℃ for 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability,in 20h,NB bacterial fluid is turbid, NA plate colony is obvious
colony morphology:	size: moderate color: white shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: translucent uplift: uplift
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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