BeNa Culture Collection
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| Culture medium | BNCC362653 |
| Description | GYM Streptomyces Agar |
| Composition | Glucose:4.0g, Yeast Extract:4.0g, Malt Extract:10.0g, CaCO3:2.0g, Agar:15.0g, pH:7.2(25℃) |
| Growth conditions | 30°C; 3–5 days; aerobic; |
| Subculture procedure | ① Prepare 1–2 of the above agar plates; ② Open in a safety cabinet, scorch the top with a酒精灯, then quickly add a drop of sterile water to cause it to crack, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, pipette approximately 200 μL onto each of the two agar plates and spread evenly; ④ Incubate the plates under the aforementioned conditions; the strain is ready for use once growth appears. |
| Storage conditions | 2–8°C |
| Safety level | 1 |
| morphology | Colonies are 1–2 mm in diameter, circular, with irregular edges; they are opaque, pale yellow on the upper surface, convex in the center, with a rough, wrinkled surface; they are dry, firm, G+ (blue-violet), rod-shaped; Purity: Pure |
| Sharing mode | Public welfare sharing |
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