Oval Brautia|375944 |BNCC

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Blautia obeum

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 48–72 h; anaerobic;
Subculture procedure	① Thaw the cryovial in a water bath (at 37°C) and shake vigorously to resuspend the bacteria; ② Disinfect the outer wall of the cryovial with an alcohol swab, then transfer it to a safety cabinet for handling; ③ Unscrew the tube cap, aspirate the entire contents, and inoculate 1–2 agar plates (approximately 200 μL per plate; for anaerobic bacteria, the medium must be placed in an anaerobic environment 24 hours in advance to remove oxygen); ④ After uniform streaking, transfer to the aforementioned culture conditions for incubation.
Storage conditions	–80°C
Safety level	1
morphology	Colonies are 0.5–1 mm in diameter, round, with smooth, well-defined edges; opaque; grayish-white on the upper surface; convex in the center; smooth surface; lustrous; moist texture; dry texture; G+ (blue-violet); coccobacilli; purity: pure
Sharing mode	Public welfare sharing