Polysaccharide Neisseria|394075 |BNCC

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Neisseria polysaccharea

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 24–48 h; 5% CO₂;
Subculture procedure	① Prepare 1–2 of the above agar plates; ② Open them in a biosafety cabinet, scorch the top with a酒精灯, then quickly add a drop of sterile water to crack the capsule, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, pipette approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the strain is ready for use once colonies appear.
Storage conditions	2–8°C
Safety level	2
morphology	Colonies are 0.5–1 mm in diameter, round, with smooth, well-defined edges; they are opaque, grayish-white on the upper surface, convex in the center, with a smooth, lustrous surface; they are moist in texture, easily lifted, G– (red), cocci; purity: pure
Sharing mode	Public welfare sharing