Staphylococcus capitis subsp. capitis|394087 |BNCC

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Staphylococcus capitis subsp. capitis LK 499

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37°C; 24–48 h; aerobic;
Subculture procedure	① Prepare 1–2 of the above agar plates; ② Open in a safety cabinet, scorch the top with a Bunsen burner, then quickly add a drop of sterile water to crack it open, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, dispense approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the culture is ready for use once colonies appear.
Storage conditions	2–8°C
Safety level	1
morphology	Colonies are 0.5–1 mm in diameter, round, with smooth, well-defined edges; they are opaque, white on the upper surface, convex in the center, with a smooth, lustrous surface; they have a moist texture, are easily lifted, are G+ (blue-violet), and are cocci; purity: pure
Sharing mode	Public welfare sharing