Lactococcus curvatus|395782 |BNCC

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Lactobacillus crispatus

Culture medium	BNCC352238
Description	MRS agar medium
Composition	Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, C₆H₈O₇.(NH₄)₂:2.0g, K₂HPO₄:2.0g, MnSO₄:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions	37°C; 48–72 h; anaerobic;
Subculture procedure	① Prepare 1–2 of the above agar plates; ② Open them in a safety cabinet, scorch the top with a酒精灯, then quickly add a drop of sterile water to crack the surface, and subsequently crush the contents with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, dispense approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the strain is ready for use once colonies appear.
Storage conditions	2–8°C
Safety level	1
morphology	Colonies are 1–2 mm in diameter, circular, with smooth, well-defined edges; they are opaque, milky white on the upper surface, convex in the center, with a smooth, lustrous surface and a moist texture. They do not produce pigments, are G+ (blue-violet), rod-shaped, and have a purity of: pure
Sharing mode	Public welfare sharing