BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ° C; 3-7 days; Strict anaerobic (liquid minced meat culture medium) |
| Subculture procedure | ① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into the liquid test tube culture medium (the culture medium is deoxygenated 24 hours in advance); ④ Transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, round, transparent, with a bright surface and a moist texture. It does not produce pigments and is G - (red) in color. It is a Bacillus subtilis strain with a purity of pure |
| Sharing mode | Public welfare sharing |
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