Pseudomonas flavida|396466 |BNCC

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Pseudomonas viridiflava 辽.P

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C; 24–48 h; aerobic
Subculture procedure	① Prepare 1–2 of the above agar plates; ② Open them in a safety cabinet, scorch the top with a酒精 lamp, then quickly add a drop of sterile water to crack them open, and subsequently crush them with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial, dissolve the bacterial powder thoroughly, then pipette 200 μL per plate and spread evenly; ④ Incubate the plates under the specified conditions; the strain is ready for use once colonies appear.
Storage conditions	2–8°C
Safety level	1
morphology	Colonies are 1–2 mm in diameter, circular, with smooth, well-defined edges; they are opaque, yellow on the upper surface, convex in the center, with a smooth, lustrous surface and a moist texture; they do not produce pigments; G– (red); bacilli; purity: pure
Sharing mode	Public welfare sharing