Candida|393669 |BNCC

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Candida sp.

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	30°C; 24–48 h; aerobic;
Subculture procedure	① Prepare 1–2 of the above agar plates; ② Open in a safety cabinet, scorch the top with a酒精灯, then quickly add a drop of sterile water to crack it open, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, dispense approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the culture is ready for use once colonies appear.
Storage conditions	2–8°C
Safety level	1
morphology	Colonies are 1–2 mm in diameter, round, with smooth, well-defined edges; they are opaque, white on the upper surface, convex in the center, with a smooth, lustrous surface; they have a moist texture, are easily lifted, are G+ (blue-violet), coccal in shape, and have a purity rating of “pure.”
Sharing mode	Public welfare sharing