Bacillus thuringiensis|396394 |BNCC

Essential Information Related Products

Bacillus velezensis

Culture medium	BNCC360815
Description	Nitrogen fixing medium
Composition	KH₂PO₄:0.2g, K₂HPO₄:0.8g, MgSO₄.7H₂O:0.2g, CaSO₄.2H₂O:0.1g, FeCl₃:Trace, Na₂MoO₄.2H₂O:Trace, Yeast Extract:0.5g, Mannitol:20.0g, Agar:15.0g, pH:7.2(25℃)
Growth conditions	37 ℃; 18-24h; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 2-4mm, circular, with irregular edges, opaque, gray white front, flat shape, smooth surface, bright surface, moist texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing