Streptomyces circinatus|393822 |BNCC

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Streptomyces anulatus

Culture medium	BNCC362653
Description	GYM Streptomyces Agar
Composition	Glucose:4.0g, Yeast Extract:4.0g, Malt Extract:10.0g, CaCO₃:2.0g, Agar:15.0g, pH:7.2(25℃)
Growth conditions	28°C; 3–5 days; aerobic;
Subculture procedure	① Prepare 1–2 of the aforementioned agar plates; ② Open the vial in a safety cabinet, scorch the top with a Bunsen burner, then quickly add a drop of sterile water to crack it open, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial; after thoroughly dissolving the bacterial powder, transfer approximately 200 μL to each of the two agar plates and spread evenly; ④ Incubate the plates under the specified conditions; the strain is ready for use once growth appears.
Storage conditions	2–8°C
Safety level	1
morphology	Colonies are 1–2 mm in diameter, circular, with irregular edges; they are opaque, grayish-white on the upper surface, convex in the center, and have a wrinkled surface. They are dry, firm, and difficult to lift or cut; G+ (blue-violet), rod-shaped; spores are spherical; purity: pure
Sharing mode	Public welfare sharing