Helicobacter pylori|395692 |BNCC

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Helicobacter pylori (Marshall et al.) Goodwin et al.

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 °; 3-4 days; 5% CO2
Subculture procedure	① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. (During the cultivation process, the agar plate should be kept moist. The agar plate can be opened in a safe cabinet and an appropriate amount of tryptone soy broth should be added.)
Storage conditions	-80 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, gray white front, raised middle, smooth surface, bright surface, moist texture, easy to pick up, G - (red), Vibrio, purity: pure
Sharing mode	Public welfare sharing