Mucous bacteria|395685 |BNCC

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Eubacterium limosum (Eggerth 1935) Prévot 1938

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic;
Subculture procedure	① Dissolve the Frozen vial in a water bath (37 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, white on the front, raised in the middle, smooth and bright on the surface, sticky and moist texture, difficult to pick/cut, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing