Oreococcus|357995 |BNCC

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Brevibacterium aurantiacum KC [NCIB 8546]

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	28°C; 24–48 h; aerobic;
Subculture procedure	① Prepare 1–2 of the above agar plates; ② Open in a safety cabinet, scorch the top with a Bunsen burner, then quickly add a drop of sterile water to rupture it, and subsequently crush it with forceps; ③ Pipette 0.5 mL of sterile water into a freeze-dried vial, dissolve the bacterial powder thoroughly, then pipette approximately 200 μL onto each of the two plates and spread evenly; ④ Incubate the plates under the specified conditions; the culture is ready for use once colonies appear.
Storage conditions	2–8°C
Safety level	1
morphology	Colonies are 0.5–1 mm in diameter, circular with smooth, well-defined edges; the surface is orange, with a raised center, smooth texture, and moist consistency; G+ (blue-violet), rod-shaped
Sharing mode	Public welfare sharing