BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C; 18-24h; Aerobic |
| Subculture procedure | ① Prepare two plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. Thoroughly dissolve powder, then inoculate two plates (200μL/Agar plate) with uniform spread; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 0.5–1 mm; circular with neat margins; opaque; pale yellow upper surface; central elevation; smooth, moist texture; easily liftable; Gram-negative (red); short rod-shaped |
| Sharing mode | Public welfare sharing |
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