BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37°C; 18-24h; Aerobic |
| Subculture procedure | ① Prepare 1-2 plates as described above; ② Open in a biosafety cabinet; follow the arrow markings on the vial caps to open the strain and reconstitution solution; ③ Add 1 mL of reconstitution solution to the lyophilized powder, cover with a rubber stopper, and shake to dissolve; ④ Pipette 200 μL of the suspension onto each of the two plates and spread evenly; ⑤ Incubate the plates under the specified conditions until colonies appear, then the strain is ready for use. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5-1 mm; circular shape with neat margins; opaque; white upper surface; central elevation; smooth, glossy surface; moist texture; easily liftable; G+ (blue-violet); cocci; purity: pure |
| Sharing mode | Public welfare sharing |
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