Pseudomonas aeruginosa|186126 |BNCC

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Pseudomonas brenneri

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Colonies are 1-2 mm in diameter, circular with entire (regular) margins, and opaque. The obverse side is grayish-white, convex (raised at the center), smooth, shiny, and moist in texture. They are easily lifted. The cells are Gram-negative (staining red), rod-shaped. Purity: Pure.
Sharing mode	Public welfare sharing