Aspergillus fumigatus|238133 |BNCC

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Aspergillus fumigatus Fresenius, anamorph

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃, 5-7 days, aerobic
Subculture procedure	① Prepare 1-2 agar plates as described above.② Sterilize the surface of the ampoule vial. Open it within a biological safety cabinet. Briefly flame the top, then immediately apply a drop of sterile water to crack it. Use sterile forceps to break it open.③ Aspirate 0.5 mL of sterile water and inject it into the freeze-dried vial. Mix thoroughly to completely resuspend the bacterial powder. Transfer 200 μL of the suspension onto each agar plate and spread evenly.④ Incubate the plates under the aforementioned culture conditions for 5-7 days.
Storage conditions	2-8 ℃
Safety level	2
morphology	The fungus exhibited white, floccose mycelium at the early stage, developing into a dark smoke-green colony with a wrinkled reverse on the agar medium at the later stage.
Sharing mode	Public welfare sharing