Staphylococcus head subspecies|277588 |BNCC

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Staphylococcus capitis subsp.‖capitis Kloos and Schleifer

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃, 18-24h, aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid medium and two agar plates.② Within a biological safety cabinet, sterilize and open the ampoule vial. Briefly flame the top, then immediately apply a drop of sterile water to crack it. Use sterile forceps to break it open.③ Aspirate 0.5 mL of the liquid medium and inject it into the freeze-dried vial. After thorough resuspension, transfer the entire volume back into the liquid medium test tube and mix well.④ Aspirate 0.2 mL of the bacterial suspension and inoculate it onto an agar plate. Spread evenly. Repeat this step to prepare a second Agar plate.⑤ Incubate both the liquid culture tube and all agar plates under the aforementioned conditions. The culture is ready for use once growth is observed.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are circular with entire margins, opaque, and convex. The surface is smooth, shiny, moist, and easily lifted. No pigment is produced. The obverse side is white (light-colored). Microscopic examination reveals Gram-positive (staining blue-violet) cocci. The culture is pure.
Sharing mode	Public welfare sharing