Soybean rhizobia|336406 |BNCC

Essential Information References Related Products

Bradyrhizobium japonicum(Kirchner)Jordan

Culture medium	BNCC353644
Description	Rhizobium Medium 1
Composition	K₂HPO₄:0.5g, MgSO₄.7H₂O:0.2g, NaCl:0.1g, Yeast Extract:1.0g, Mannitol:10.0g, Congo Red:0.025g, Agar:15.0g, pH:7.2±0.2(25℃)
Growth conditions	30 ℃; 3-4 days; aerobic;
Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, mix thoroughly and thoroughly; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, light pink on the front, raised in the middle, smooth and bright on the surface, sticky and moist texture, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing