Broomycota|112791 |BNCC

Essential Information Related Products

Cylindrocladium sp.

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	25-28 ℃; 5-7 days; Aerobic
Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the plate and onto the surface of the agar; ⑤ Cultivate the flat plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	-80 ℃
Safety level	1
morphology	A small filamentous fungus that forms distinct colonies on comprehensive PDA medium. The mycelium is white, cotton-like in texture, dense, and vigorous, spreading concentrically toward the edges of the plate. No spore production is observed.
Sharing mode	Public welfare sharing