Cicada Flower 1-4 (Cicada Penicillium)|114807 |BNCC

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Cordyceps cicadae

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃; 5-7 days; aerobic
Subculture procedure	① Prepare 1-2 tablets mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the agar plate and onto the surface of the agar; ⑤ Cultivate the flat agar plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	Liquid nitrogen
Safety level	1
morphology	A small filamentous fungus forming distinct, white colonies on comprehensive PDA medium. The mycelium is dense and vigorous, spreading toward the agar plate edges. The reverse side of the medium shows a pale yellow coloration.
Sharing mode	Public welfare sharing