Salt denitrifying Bacillus subtilis|132612 |BNCC

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Virgibacillus halodenitrificans

Culture medium	BNCC353940
Description	3.5% Sodium Chloride Agar Medium
Composition	Beef Extract:3.0g, Peptone:10.0g, NaCl:35.0g, Agar:15.0g, pH:7.2-7.4(25℃)
Growth conditions	30 ℃, 18-24h, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, yellow on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing