Youthful Bifidobacterium|134301 |BNCC

Essential Information References Related Products

Bifidobacterium adolescentis

Culture medium	BNCC352254
Description	BBL agar medium
Composition	Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic
Subculture procedure	① Prepare two of the aforementioned agar plates (pre-equilibrated in an anaerobic environment for 24 hours).② Surface-sterilize the ampoule, open it inside a biosafety cabinet, flame the top using an alcohol lamp, and promptly apply sterile water to crack it open. Then, carefully crush the ampoule using sterile forceps.③ Aspirate 0.5 mL of sterile water (pre-equilibrated in an anaerobic environment for 24 hours) and inject it into the lyophilized vial. After fully dissolving the bacterial powder, transfer the suspension onto the two prepared plates, distributing 200 µL per Agar plate, and spread evenly.④ Incubate the plates under the specified culture conditions. The bacterial strain is ready for use once growth is observed.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are 0.5-1 mm in diameter, circular with entire edges, opaque, convex in the center, and have a dull, smooth, moist surface. They are easily picked, Gram-positive (blue-violet), and consist of rod-shaped cells.
Sharing mode	Public welfare sharing