Radiation resistant cocci|136835 |BNCC

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Deinococcus radiodurans

Culture medium	BNCC360866
Description	Peptone Yeast extract Glucose
Composition	Peptone:20.0g, Glucose:5.0g, Yeast Extract Powder:10.0g, NaCl:0.08g, Cysteine Hydrochloride(C₃H₇NO₂S.HCl):0.5g, CaCl₂:0.008g, MgSO₄:0.008g, K₂HPO₄:0.04g, KH₂PO₄:0.04g, NaHCO₃:0.4g, pH:6.0±0.1(25℃)
Growth conditions	30 ℃; 24-48 hours; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, pink front, smooth surface, bright surface, difficult to pick, does not produce pigments, G+(blue purple), cocci (quadruple arrangement), purity: pure
Sharing mode	Public welfare sharing