BeNa Culture Collection
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| Culture medium | BNCC360866 |
| Description | Peptone Yeast extract Glucose |
| Composition | Peptone:20.0g, Glucose:5.0g, Yeast Extract Powder:10.0g, NaCl:0.08g, Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, CaCl2:0.008g, MgSO4:0.008g, K2HPO4:0.04g, KH2PO4:0.04g, NaHCO3:0.4g, pH:6.0±0.1(25℃) |
| Growth conditions | 30 ℃; 24-48 hours; aerobic; |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, pink front, smooth surface, bright surface, difficult to pick, does not produce pigments, G+(blue purple), cocci (quadruple arrangement), purity: pure |
| Sharing mode | Public welfare sharing |
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