BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC369665 |
| Description | Tryptic Soy Broth (TSB) with 10μg/mL Ceftazidime |
| Composition | Tryptone:17.0g, Soytone:3.0g, NaCl:5.0g, K2HPO4:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1 tube containing 5-10mL liquid medium or sterile water and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The culture is ready for use once the strain grows. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 1-2mm, circular, with neat edges; opaque, pale yellow on the upper surface; centrally raised, smooth surface, moist texture; G+ (blue-violet), cocci; Purity: Pure |
| Sharing mode | Public welfare sharing |
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