BeNa Culture Collection
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| Culture medium | BNCC360812 |
| Description | Concentrated sugar Chatelier agar |
| Composition | Sucrose:200.0g, NaNO3:3.0g, MgSO4.7H2O:0.5g, KCl:0.5g, FeSO4.4H2O:0.01g, K2HPO4:1.0g, Agar:15.0g, pH:6.0-6.5(25℃) |
| Growth conditions | 28 ℃; 7-10 days; aerobic |
| Subculture procedure | ① Prepare the above 2 plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Spread and grow, producing gray green spores, with gray green on the front and dark on the back |
| Sharing mode | Public welfare sharing |
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