Pseudomonas aeruginosa|139204 |BNCC

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Bacteroides uniformis

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic;
Subculture procedure	① Prepare 1-2 fresh agar plates as specified above (pre‑conditioned in an anaerobic environment for 24 h to remove oxygen).② In a biosafety cabinet, open the freeze‑dried vial, briefly flame the top with an alcohol burner, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of liquid medium or sterile water (pre‑conditioned in an anaerobic environment for 24 h to remove oxygen) into the freeze‑dried vial, thoroughly dissolve the contents, and mix well.④ Transfer 0.2 mL of the resulting bacterial suspension onto an agar plate, spread evenly, and repeat to obtain two separate plates.⑤ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white coloration on the surface, have a raised center, and are smooth and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli).
Sharing mode	Public welfare sharing