BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 48-72h; anaerobic; |
| Subculture procedure | ① Prepare 1-2 fresh agar plates as specified above (pre‑conditioned in an anaerobic environment for 24 h to remove oxygen).② In a biosafety cabinet, open the freeze‑dried vial, briefly flame the top with an alcohol burner, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of liquid medium or sterile water (pre‑conditioned in an anaerobic environment for 24 h to remove oxygen) into the freeze‑dried vial, thoroughly dissolve the contents, and mix well.④ Transfer 0.2 mL of the resulting bacterial suspension onto an agar plate, spread evenly, and repeat to obtain two separate plates.⑤ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white coloration on the surface, have a raised center, and are smooth and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). |
| Sharing mode | Public welfare sharing |
KMS11(KMS11)
BNCC340591
TJ905(TJ905)
BNCC339987
OCI-AML-5(OCI-AML-5)
BNCC341619
NCI-H2286(NCI-H2286)
BNCC342561
Py230(Py230)
BNCC378460
SN-12C(SN-12C)
BNCC396361