Legionella dumoffii|370725 |BNCC

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Fluoribacter dumoffii

Culture medium	BNCC360928
Description	Buffered Charcoal Yeast Extract Agar
Composition	Yeast Extract Powder:10.0g, Activated Charcoal:2.0g, Agar:13.0g, α-Ketoglutarate Monopotassium Salt(C₅H₅KO₅):1.0g, ACES Buffer:10.0g, Potassium Hydroxide(KOH):2.8g, Ferric Pyrophosphate(Fe₄(P₂O₇)₃):0.25g
Growth conditions	37°C; 24-48h; aerobic;
Subculture procedure	① Prepare 1-2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush contents with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	2
morphology	Colony diameter: 1-2 mm; circular with irregular margins; upper surface white, centrally convex, smooth, moist texture, easily liftable; non-pigmented, Gram-negative (red), bacillus; purity: pure
Sharing mode	Public welfare sharing