BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360928 |
| Description | Buffered Charcoal Yeast Extract Agar |
| Composition | Yeast Extract Powder:10.0g, Activated Charcoal:2.0g, Agar:13.0g, α-Ketoglutarate Monopotassium Salt(C5H5KO5):1.0g, ACES Buffer:10.0g, Potassium Hydroxide(KOH):2.8g, Ferric Pyrophosphate(Fe4(P2O7)3):0.25g |
| Growth conditions | 37°C; 24-48h; aerobic; |
| Subculture procedure | ① Prepare 1-2 plates as described above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush contents with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 1-2 mm; circular with irregular margins; upper surface white, centrally convex, smooth, moist texture, easily liftable; non-pigmented, Gram-negative (red), bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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