BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 48-72h; 5% CO₂ |
| Subculture procedure | ① Prepare 1-2 fresh plates as described above; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL sterile water into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension onto the Agar plate and spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the strain is ready for use once colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 2 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat, opaque edges; grayish-white upper surface; central elevation; smooth, glossy surface; moist texture; Gram-negative (red); cocci; Purity: Pure |
| Sharing mode | Public welfare sharing |
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