Salmonella enterica subsp. cinerea serotype|192092 |BNCC

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Salmonella enterica subsp. enterica serotype Bonariensis

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	Colony diameter 1-2 mm, circular with entire margin, opaque, light yellow on the obverse, convex in the center, smooth and glistening surface, moist texture, easily picked, Gram-negative (red), rod-shaped, purity: pure.
Sharing mode	Public welfare sharing