Bacillus licheniformis|185404 |BNCC

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Bacillus licheniformis

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃; 18-24h; aerobic;
Subculture procedure	① Prepare 1-2 plates as described above.② Within a biosafety cabinet, open the vial and flame the top briefly with an alcohol lamp, then immediately add sterile water to break the seal. Use forceps to crush the vial.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2 mm,Shape: Irregular,Margin: Irregular,Transparency: Opaque,Color: Pale yellow,Elevation: Flat,Surface: Rough and dull,Texture: Dry
Sharing mode	Public welfare sharing