Lactococcus lactis subsp. lactis|185907 |BNCC

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Lactococcus lactis subsp. lactis

Culture medium	BNCC352238
Description	MRS agar medium
Composition	Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, C₆H₈O₇.(NH₄)₂:2.0g, K₂HPO₄:2.0g, MnSO₄:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Subculture procedure	① Prepare 1-2 plates as described above, along with sterile water (pre‑equilibrated in an anaerobic environment for 24 h to remove oxygen).② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol lamp, then immediately add sterile water to break the seal and crush the vial using sterile forceps.③ Aspirate 0.5 mL of the pre‑treated sterile water into the freeze‑dried vial, thoroughly dissolve the bacterial powder, then transfer 200 μL of the suspension onto each Agar plate and spread evenly.④ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies measure 1-2 mm in diameter, are circular with irregular margins, and are opaque. They appear white on the surface (obverse) and golden yellow on the reverse side, with a raised center. The colonies are smooth, glossy, and moist in texture, making them easy to lift. No pigment is produced. The organisms are Gram-positive (G⁺, appearing blue-violet).
Sharing mode	Public welfare sharing