Occasional subspecies of sporadically branched bacteria|186102 |BNCC

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Mycobacterium fortuitum subsp. fortuitum

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 48-72h; aerobic;
Subculture procedure	① Prepare 1-2 plates as specified above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol lamp, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies measure 0.5-1 mm in diameter, exhibit an irregular shape and irregular margins, and are opaque. They appear pale yellow on the surface, display a flat morphology, and have a rough surface with a dry texture. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli).
Sharing mode	Public welfare sharing