Bacillus subtilis subspecies|191554 |BNCC

BeNa Culture Collection

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Bacillus subtilis subspecies-BNCC
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  • Bacillus subtilis subsp. subtilis-BNCC

Bacillus subtilis subsp. subtilis

  • Price： $ 186
  • number：BNCC191554
  • MPs：
    Size: 2-4 mm,Shape: Circular,Margin: Irregular,Transparency: Opaque,Color: Grayish-white,Elevation: Flat,Surface: Rough,Texture: Dry
  • Packing：Freeze dried pellet; Agar Slant; Bacterial suspension; Agar plate
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Essential Information Related Products
Bacillus subtilis subsp. subtilis
Culture medium BNCC352239
Description Nutrient Agar
Composition Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions 37 ℃; 18-24h; aerobic
Subculture procedure ① Prepare 1-2 plates as specified above.② Open the vial in a biosafety cabinet, briefly flame the top with an alcohol lamp, and immediately add sterile water to break the seal. Then crush the vial using sterile forceps.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, fully dissolve the bacterial powder, then transfer approximately 200 µL of the suspension onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The strain can be used once growth appears.
Storage conditions 2-8 ℃
Safety level 1
morphology Size: 2-4 mm,Shape: Circular,Margin: Irregular,Transparency: Opaque,Color: Grayish-white,Elevation: Flat,Surface: Rough,Texture: Dry
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