Grape juice contains spore forming Hansenula yeast|193162 |BNCC

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Hanseniaspora uvarum

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	28 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They appear white on the surface, have a raised center, and are smooth, glossy, and moist in texture. No pigment is produced. The organisms are Gram-positive (G⁺, appearing blue-violet) and spherical (cocci). Purity: pure.
Sharing mode	Public welfare sharing