Pusa rhizobia|231877 |BNCC

BeNa Culture Collection

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Pusa rhizobia-BNCC
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  • Rhizobium pusense-BNCC

Rhizobium pusense

  • Price： $ 215
  • number：BNCC231877
  • MPs：
    The colonies measure 2-4 mm in diameter, are circular with regular margins, and are translucent. They exhibit a light pink pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure.
  • Packing：Freeze dried pellet; Agar Slant; Bacterial suspension; Agar plate
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Rhizobium pusense
Culture medium BNCC353644
Description Rhizobium Medium 1
Composition K2HPO4:0.5g, MgSO4.7H2O:0.2g, NaCl:0.1g, Yeast Extract:1.0g, Mannitol:10.0g, Congo Red:0.025g, Agar:15.0g, pH:7.2±0.2(25℃)
Growth conditions 28 ℃; 24-48 hours; aerobic
Subculture procedure ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions 2-8 ℃
Safety level 1
morphology The colonies measure 2-4 mm in diameter, are circular with regular margins, and are translucent. They exhibit a light pink pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure.
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