Beauveria bassiana|117565 |BNCC

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Beauveria bassiana (Bals.-Criv.) Vuill.

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃; 3-5 days; aerobic
Subculture procedure	① Prepare 1 test tube containing 5-10 mL of liquid culture medium or sterile water and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	The small filamentous fungus forms distinct colonies on comprehensive PDA medium, exhibiting white, dense, and low-lying mycelium. The reverse side of the medium shows a pale yellow color.
Sharing mode	Public welfare sharing