BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 fresh agar plates as described above.② Working in a biosafety cabinet, open the freeze-dried vial and briefly flame the top with an alcohol burner. Immediately add sterile water to the vial to break the seal, then use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, thoroughly dissolve the contents, and mix well.④ Transfer 0.2 mL of the resulting bacterial suspension onto an agar plate, spread evenly using a sterile spreader, and repeat to inoculate a second Agar plate.⑤ Incubate the plates under the specified culture conditions. The strain is ready for use once growth is observed. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure. |
| Sharing mode | Public welfare sharing |
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