Klebsiella pneumoniae subsp. pneumoniae|330357 |BNCC

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Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Trevisan

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	37 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 fresh agar plates as described above.② Working in a biosafety cabinet, open the freeze-dried vial and briefly flame the top with an alcohol burner. Immediately add sterile water to the vial to break the seal, then use sterile forceps to crush the vial.③ Aspirate 0.5 mL of sterile water into the freeze-dried vial, thoroughly dissolve the contents, and mix well.④ Transfer 0.2 mL of the resulting bacterial suspension onto an agar plate, spread evenly using a sterile spreader, and repeat to inoculate a second Agar plate.⑤ Incubate the plates under the specified culture conditions. The strain is ready for use once growth is observed.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure.
Sharing mode	Public welfare sharing